Journal: Viruses

Article Title: Discovery of Three Toxic Proteins of Klebsiella Phage fHe-Kpn01

doi: 10.3390/v12050544

Figure Lengend Snippet: Growth curves of Klebsiella pneumoniae strains #5504 ( A ) and #6326 ( B ) infected with fHe-Kpn01. Bacteria were cultured with different initial multiplicities of infections (MOIs) in liquid LB-medium at 37 °C. Each data point represents the average optical density (OD) at 600 nm for four to five replicates and error bars represent the standard deviation (SD).

Article Snippet: The K. pneumoniae strains used in this work are described in and were mostly obtained from the hospital district of Helsinki and Uusimaa laboratories (HUSLAB), Finland.

Techniques: Infection, Bacteria, Cell Culture, Standard Deviation

Journal: Infection and Drug Resistance

Article Title: Synergistic Effect and Mechanism of Plumbagin with Gentamicin Against Carbapenem-Resistant Klebsiella pneumoniae

doi: 10.2147/IDR.S265753

Figure Lengend Snippet: Plumbagin potentiates antibacterial activity of gentamicin against CRKp. ( A ) Susceptibility test to imipenem of antibiotics-susceptible strain of Klebsiella pneumoniae CCTCC AB 2010163 (left) and CRKp strain ATCC BAA-1705 (right) on LB agar plates. ( B ) Susceptibility test to gentamicin of ATCC BAA-1705 (left) and its induced gentamicin-resistant strain (right) on LB agar plates. ( C ) Heatmap profile of synergistic effect of herbal compounds with gentamicin. Red, no synergistic effect; green, low synergistic effect; and purplish red, high synergistic effect. ( D ) The inhibition of combination treatments against the CRKp. ( E ) The inhibition of combination treatments against antibiotics-susceptible strain of Klebsiella pneumoniae . ( F ) Growth curves of CRKp with different treatments. * P <0.05, ** P <0.01, *** P <0.001, and **** P <0.0001 compared with gentamicin monotherapy.

Article Snippet: The antibiotics-susceptible strain of Klebsiella pneumoniae CCTCC AB 2010163 was obtained from China Center for Type Culture Collection (CCTCC), Wuhan, China.

Techniques: Activity Assay, Inhibition

Journal: Repetitorium Krankenhaushygiene und hygienebeauftragter Arzt

Article Title: Spezielle Erreger und Infektionen

doi: 10.1007/978-3-642-36864-6_14

Figure Lengend Snippet:

Article Snippet: Die Verbreitung von 3MRGN K. pneumoniae ist in lokalen Ausbrüchen in der Regel ebenfalls klonal, es ist jedoch unklar ob eine überregionale Verbreitung, die mit Einrichtungen der stationären oder ambulanten Patientenversorgung assoziiert ist, erfolgt.

Techniques:

Journal: Repetitorium Krankenhaushygiene und hygienebeauftragter Arzt

Article Title: Spezielle Erreger und Infektionen

doi: 10.1007/978-3-642-36864-6_14

Figure Lengend Snippet:

Article Snippet: Die Verbreitung von 3MRGN K. pneumoniae ist in lokalen Ausbrüchen in der Regel ebenfalls klonal, es ist jedoch unklar ob eine überregionale Verbreitung, die mit Einrichtungen der stationären oder ambulanten Patientenversorgung assoziiert ist, erfolgt.

Techniques: In Vitro

Journal: Repetitorium Krankenhaushygiene und hygienebeauftragter Arzt

Article Title: Spezielle Erreger und Infektionen

doi: 10.1007/978-3-642-36864-6_14

Figure Lengend Snippet:

Article Snippet: Die Verbreitung von 3MRGN K. pneumoniae ist in lokalen Ausbrüchen in der Regel ebenfalls klonal, es ist jedoch unklar ob eine überregionale Verbreitung, die mit Einrichtungen der stationären oder ambulanten Patientenversorgung assoziiert ist, erfolgt.

Techniques:

Journal: Fems Immunology and Medical Microbiology

Article Title: Biofilm formation of Klebsiella pneumoniae on urethral catheters requires either type 1 or type 3 fimbriae

doi: 10.1111/j.1574-695X.2012.00965.x

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: A modification of a previously described method was used to determine the orientation of the K. pneumoniae fim switch ( ; Struve et al ., 2008 ).

Techniques: Plasmid Preparation, Clone Assay

Journal: Fems Immunology and Medical Microbiology

Article Title: Biofilm formation of Klebsiella pneumoniae on urethral catheters requires either type 1 or type 3 fimbriae

doi: 10.1111/j.1574-695X.2012.00965.x

Figure Lengend Snippet: Expression of type 1 (Red) and type 3 (Green) fimbriae by individual Klebsiella pneumoniae C3091 cells grown as a biofilm on urinary tract catheters (a) or planktonically in urine (b) using type 1 fimbriae specific antibodies and type 3 fimbriae specific antibodies. Black cells are non-fimbriated cells. Note that during biofilm formation on urinary catheters each individual cell is expressing either type 1 or type 3 fimbriae not both. Scale bars denote 20 μm.

Article Snippet: A modification of a previously described method was used to determine the orientation of the K. pneumoniae fim switch ( ; Struve et al ., 2008 ).

Techniques: Expressing

Journal: Scientific Reports

Article Title: PathCrisp : an innovative molecular diagnostic tool for early detection of NDM-resistant infections

doi: 10.1038/s41598-024-84832-z

Figure Lengend Snippet: Validation of designed oligos for NDM variants: ( a ) testing with genomic DNA of K. pneumoniae : the genomic DNA of K. pneumoniae , positive for NDM-1 (obtained from Vircell), is being used to test the designed LAMP primers and sgRNA. Our designed guide and primers can detect NDM-1. ( b ) specificity testing with negative controls: different known negative genomic DNAs are being used to test the specificity of the PathCrisp assay, with previously used K. pneumoniae (NDM-1) as assay control. The assay was repeated twice: once with Bst1.0 (without UDG) depicted as circles in the graph, and a second time with BST2.0 (with UDG) depicted as diamonds in the graph. The average value of each sample is represented as a bar graph, while individual values are marked either as circles or diamonds. Grey dotted lines represent the cutoff to consider if the sample is positive, which is the average of RFU of LAMP-NTC plus 3 times the standard deviation. ( c ) Testing with carbapenem-resistant E. coli isolates: sixteen carbapenem-resistant E. coli isolates from patient samples were tested with designed LAMP primers and sgRNA. VIR-5, VIR-8 and VIR-9 were negative for NDM, consistent with the previous report. The NDM as per previous sequencing data is mentioned for the samples. In each assay, the negative control for LAMP is no-template control, depicted as LAMP-NTC. For detection controls, negative control, with no LAMP product, is shown as Det. negative in the graph. For positive control, pJET1.2_NDM was used, depicted as Det. positive.

Article Snippet: Fig. 2 Validation of designed oligos for NDM variants: ( a ) testing with genomic DNA of K. pneumoniae : the genomic DNA of K. pneumoniae , positive for NDM-1 (obtained from Vircell), is being used to test the designed LAMP primers and sgRNA.

Techniques: Control, Standard Deviation, Sequencing, Negative Control, Positive Control

Journal: PLoS ONE

Article Title: Impact of acetolactate synthase inactivation on 1,3-propanediol fermentation by Klebsiella pneumoniae

doi: 10.1371/journal.pone.0200978

Figure Lengend Snippet: Bacterial strains and plasmids.

Article Snippet: K . pneumoniae ΔALS , K . pneumoniae CCTCC M2011075, ΔALS, aac(3)IV , This work.

Techniques: Plasmid Preparation, TA Cloning, Expressing

Journal: Life

Article Title: Resistance to Some New Drugs and Prevalence of ESBL- and MBL-Producing Enterobacteriaceae Uropathogens Isolated from Diabetic Patients

doi: 10.3390/life12122125

Figure Lengend Snippet: Prevalence of enteric uropathogens from diabetic patients ( n = 910).

Article Snippet: K. pneumoniae ( n = 91) , MBL , 61 (67.03%) , , , , , , , 64 (70.3%) , 27 (29.67%) , 00 , 64 (70.3%) , 27 (29.67%) , 0.

Techniques:

Journal: Life

Article Title: Resistance to Some New Drugs and Prevalence of ESBL- and MBL-Producing Enterobacteriaceae Uropathogens Isolated from Diabetic Patients

doi: 10.3390/life12122125

Figure Lengend Snippet: Antimicrobial resistance patterns of Enterobacteriaceae uropathogens.

Article Snippet: K. pneumoniae ( n = 91) , MBL , 61 (67.03%) , , , , , , , 64 (70.3%) , 27 (29.67%) , 00 , 64 (70.3%) , 27 (29.67%) , 0.

Techniques:

Journal: Life

Article Title: Resistance to Some New Drugs and Prevalence of ESBL- and MBL-Producing Enterobacteriaceae Uropathogens Isolated from Diabetic Patients

doi: 10.3390/life12122125

Figure Lengend Snippet: Resistance of the Enterobacteriaceae uropathogen against colistin. The isolates with MIC ≤ 2μg/ mL were considered as intermediate, and isolates with ≥4 µg/mL were taken as resistant. The criteria for sensitivity is not mentioned in CLSI (2021).

Article Snippet: K. pneumoniae ( n = 91) , MBL , 61 (67.03%) , , , , , , , 64 (70.3%) , 27 (29.67%) , 00 , 64 (70.3%) , 27 (29.67%) , 0.

Techniques:

Journal: Life

Article Title: Resistance to Some New Drugs and Prevalence of ESBL- and MBL-Producing Enterobacteriaceae Uropathogens Isolated from Diabetic Patients

doi: 10.3390/life12122125

Figure Lengend Snippet: Detection of ESBL production using phenotypic method, manual PCR, and microarray assay.

Article Snippet: K. pneumoniae ( n = 91) , MBL , 61 (67.03%) , , , , , , , 64 (70.3%) , 27 (29.67%) , 00 , 64 (70.3%) , 27 (29.67%) , 0.

Techniques: Microarray, Polymerase Chain Reaction

Journal: Life

Article Title: Resistance to Some New Drugs and Prevalence of ESBL- and MBL-Producing Enterobacteriaceae Uropathogens Isolated from Diabetic Patients

doi: 10.3390/life12122125

Figure Lengend Snippet: Characterization of carbapenemases into MBL and KPC on phenotypic basis and detection of carbapenemases encoding genes ( n = 250).

Article Snippet: K. pneumoniae ( n = 91) , MBL , 61 (67.03%) , , , , , , , 64 (70.3%) , 27 (29.67%) , 00 , 64 (70.3%) , 27 (29.67%) , 0.

Techniques: Microarray

Journal: Life

Article Title: Resistance to Some New Drugs and Prevalence of ESBL- and MBL-Producing Enterobacteriaceae Uropathogens Isolated from Diabetic Patients

doi: 10.3390/life12122125

Figure Lengend Snippet: Resistance to a new regimen, cefiderocol (FDC) and eravacycline (ERV), in CRE isolates ( n = 250).

Article Snippet: K. pneumoniae ( n = 91) , MBL , 61 (67.03%) , , , , , , , 64 (70.3%) , 27 (29.67%) , 00 , 64 (70.3%) , 27 (29.67%) , 0.

Techniques: